Dipole-Dipole Interaction

Dipole-Dipole interactions occur between molecules that have permanent dipoles; these molecules are also referred to as polar molecules.

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#Unit 13. Methods in Biology

AFLP uses restriction enzyme-digested genomic DNA as template for PCR amplification using primers that contain the restriction enzyme recognition sites plus a number of, usually 2-3, arbitrary nucleotides.

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#Unit 13. Methods in Biology

AFLPs are faster, less labour intensive and provide more information than RFLPs, and they are highly reproducible, which is a great advantage over RAPDs.

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#Unit 13. Methods in Biology

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#Unit 13. Methods in Biology

An AFLP is detected as a differential mobility of an amplified DNA fragment; AFLPs, therefore, behave as codominant markers during inheritance.

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#Unit 13. Methods in Biology

Linker and/or adapter molecules can be joined to the cut ends. Linkers are short, chemically synthesized, double-stranded oligonucleotides, which contain within them one or more restriction endonuclease sites, e.g., linker 5'CCGAATTCGG (only one strand of the linker is shown here) contains one EcoRI site. 

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#Unit 13. Methods in Biology

Linkers are fused with blunt-ended DNA fragments; cleavage of the linker with the appropriate restriction enzyme creates suitable cohesive protruding ends.