AP activates a  voltage gated Ca2+ channel in the T-tubule membrane, it triggers the opening of a Ca2+ -release channel in the closely associated SR membrane

TLS Online TPP Program

#Id: 4656

#Unit 13. Methods in Biology

Carboxyfluorescein succinimidyl ester (CFSE) is more correctly named carboxyfluorescein diacetate succinimidyl ester (CFDASE) very commonly used to detect state of cell 

TLS Online TPP Program

#Id: 4657

#Unit 13. Methods in Biology

(CFSE) or carboxyfluorescein diacetate succinimidyl ester (CFDASE) is nontoxic to cells hence retained till many cycles

TLS Online TPP Program

#Id: 4658

#Unit 13. Methods in Biology

CFSE labeling amount of fluorescence emitted is cut in half each time the cell divides.

TLS Online TPP Program

#Id: 4659

#Unit 13. Methods in Biology

The 51Cr Release Assay is used to measure Cell death not apoptosis

TLS Online TPP Program

#Id: 4660

#Unit 13. Methods in Biology

Normal cells treated with sodium chromate allowed to absorb and induced to death signals as T-CTLs Death of the target cells is indicated by the release of 51Cr into the supernatant

TLS Online TPP Program

#Id: 4661

#Unit 13. Methods in Biology

FITC binds to Annexin V to phosphatidyl serine which are found on apoptotic cells, analyzed by FACS