Five types of constraints affect the stability of an  helix: 

(1) the intrinsic propensity of an amino acid residue to form an  helix; 

(2) the interactions between R groups, particularly those spaced three (or four) residues apart; 

(3) the bulkiness of adjacent R groups; 

(4) the occurrence of Pro and Gly residues; and 

(5) interactions between amino acid residues at the ends of the helical segment and the electric dipole inherent to the  helix.

TLS Online TPP Program

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#Unit 3. Fundamental Processes

The GATC sites at the origin remain hemimethylated for -13 minutes after replication.

TLS Online TPP Program

#Id: 3133

#Unit 3. Fundamental Processes

Initiation at oriC requires the sequential assembly of a large protein complex on the membrane.

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#Id: 3134

#Unit 3. Fundamental Processes

The ability of a plasmid relying upon oriC to replicate in dam- E. coli depends on its state  of methylation.

TLS Online TPP Program

#Id: 3159

#Unit 3. Fundamental Processes

 Eukaryotic cells have many DNA polymerases. The replication enzymes operate with high fidelity. Except  for the β enzyme, the repair enzymes all have low fidelity.

TLS Online TPP Program

#Id: 3160

#Unit 3. Fundamental Processes

Replication enzymes have large structures, with separate subunits for different activities. Repair enzymes have much simpler structures.

TLS Online TPP Program

#Id: 3161

#Unit 3. Fundamental Processes

The conserved function of the replication components extends to the clamp loader and processivity clamp as well other functions of the replisome.

The roles of RFC and PCNA are analogous to the E. coli γ clamp loader and β2 processivity unit