Certain side-chain conformations are energetically favourable, these side chain confirmations are called as Rotamers.

TLS Online TPP Program

#Id: 2803

#Unit 13. Methods in Biology

In NPLC the most popular stationary phase is an alkylamine bonded to silica. The mobile phase is generally an organic solvent such as hexane, heptane, dichloromethane or ethyl acetate.

TLS Online TPP Program

#Id: 2804

#Unit 13. Methods in Biology

In NPLC solvents form an elutropic series based on their polarity. Such a series in

TLS Online TPP Program

#Id: 2805

#Unit 13. Methods in Biology

 In NPLC order of elution of analytes is such that the least polar is eluted first and the most polar last.

TLS Online TPP Program

#Id: 2806

#Unit 13. Methods in Biology

The main applications of normal-phase liquid chromatography are its use to separate

analytes that have low water solubility and those that are not amenable to reversed phase liquid chromatography.

TLS Online TPP Program

#Id: 2807

#Unit 13. Methods in Biology

The pH of the buffer selected as eluent should be at least one pH unit above or below the isoionic point of the analytes.

TLS Online TPP Program

#Id: 2808

#Unit 13. Methods in Biology

Reversed-phase liquid chromatography form of liquid chromatography, which has many similarities with hydrophobic interaction chromatography (HIC).