Similarly, if the inhibitor binds to a different site from the substrate and reduces the affinity of the enzyme for the substrate without altering the reaction characteristics of that substrate which does bind, then hyperbolic competitive inhibition results

TLS Online TPP Program

#Id: 1846

#Unit 3. Fundamental Processes

Molecular genetic techniques are used to make a cDNA encoding a recombinant protein to which is fused a short sequence of amino acids called an epitope tagging.

TLS Online TPP Program

#Id: 1847

#Unit 3. Fundamental Processes

Two commonly used epitope tags are FLAG, which encodes the amino acid sequence DYKDDDDK (single-letter amino acid code), and myc, which encodes the sequence EQKLISEEDL.

TLS Online TPP Program

#Id: 1848

#Unit 3. Fundamental Processes

TLS Online TPP Program

#Id: 1849

#Unit 3. Fundamental Processes

Fluorochrome-coupled monoclonal antibodies to the FLAG or myc epitopes can then be used to detect the recombinant protein in the cell.

Alexa- 488-labeled antibody -emit green light 

Alexa-568-labeled antibody- emit red light

TLS Online TPP Program

#Id: 1850

#Unit 3. Fundamental Processes

TLS Online TPP Program

#Id: 1851

#Unit 3. Fundamental Processes