In free CDK2, the A-loop blocks access of protein substrates to the γ phosphate of the bound ATP.

TLS Online TPP Program

#Id: 8768

#Unit 1. Molecules and their Interaction Relevant to Biology

TLS Online TPP Program

#Id: 8769

#Unit 1. Molecules and their Interaction Relevant to Biology

TLS Online TPP Program

#Id: 8770

#Unit 1. Molecules and their Interaction Relevant to Biology

                                                                                                                                                                                                                                                                      

TLS Online TPP Program

#Id: 8771

#Unit 1. Molecules and their Interaction Relevant to Biology

TLS Online TPP Program

#Id: 8772

#Unit 1. Molecules and their Interaction Relevant to Biology

van’t Hoff plot is used measure rate of unfolding of proteins

TLS Online TPP Program

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#Unit 1. Molecules and their Interaction Relevant to Biology

Techniques for Measuring Stability

Absorbance (e.g. Trp, Tyr) 

Fluorescence (Trp)-difference in emission max & intensity.

CD (far or near UV) - (2o or 3o) 

NMR 

DSC (calorimetry) 

Urea gradient gels - difference in the migrating rates between F and U.

Catalytic activity 

Chromophoric or fluorophoric probes/FRET