TLS Online TPP Program

#Id: 2835

#Unit 13. Methods in Biology

GST tagged protein are separated by using Glutathione (a tripeptide with a g-peptide linkage, g -Glu-Cys-Gly), displacing the bound GST moiety.

TLS Online TPP Program

#Id: 2836

#Unit 13. Methods in Biology

Maltose-Binding Protein (MBP) is also used for fusion tag for protein which are extremely GST

TLS Online TPP Program

#Id: 2837

#Unit 13. Methods in Biology

Maltose-Binding Protein (MBP) One-step purification can be achieved using amylose resin

TLS Online TPP Program

#Id: 2838

#Unit 13. Methods in Biology

Poly-Histidine Tag (His-tag) is also used as tag to separate protein

TLS Online TPP Program

#Id: 2839

#Unit 13. Methods in Biology

These histidine's are separated by using Nickel-chelate-nitrilotriacetate (Ni-NTA) chromatography column.

TLS Online TPP Program

#Id: 2840

#Unit 13. Methods in Biology

These are His-tag subsequently removed by cleavage with a reagent such as cyanogen bromide and the protein of interest purified by protein biochemical methods such as chromatography and polyacrylamide gel electrophoresis.