Disulfide bonds are believed to increase the stability of the native state by decreasing the conformational entropy of the unfolded state due to the conformational constraints imposed by cross-linking (i. e. decreasing the free energy of the unfolded state).  

TLS Online TPP Program

#Id: 2609

#Unit 13. Methods in Biology

RACE can provide the sequence of an RNA transcript from a small known sequence within the transcript to the 5' end (5' RACE-PCR) or 3' end (3' RACE-PCR) of the RNA. 

TLS Online TPP Program

#Id: 2610

#Unit 13. Methods in Biology

RACE is sometimes called one-sided PCR or anchored PCR. 

TLS Online TPP Program

#Id: 2611

#Unit 13. Methods in Biology

RACE can be used to amplify unknown 5' (5'-RACE) or 3' (3'-RACE) parts of RNA molecules where part of the RNA sequence is known and targeted by a GSP gene-specific primer.

TLS Online TPP Program

#Id: 2612

#Unit 13. Methods in Biology

Our on-going research indicates that RLMRACE, PPM-RACE, and qRT-PCR are very effective in the verification of sequences of miRNA targets obtained by Degradome sequencing. 

TLS Online TPP Program

#Id: 2613

#Unit 13. Methods in Biology

The protocol for RLM-RACE, PPMRACE, and qRT-PCR is rapid, effective, cheap, and can be completed within 2–3 day.

TLS Online TPP Program

#Id: 2614

#Unit 13. Methods in Biology

To more accurately validate the predicted target genes of miRNAs and exactly determine the cleavage sites within the targets, an integrated strategy comprising RLM-RACE, Poly(A) Polymerase-Mediated (PPM)-RACE, and qRT-PCR was developed.