Techniques for Measuring Stability

Absorbance (e.g. Trp, Tyr) 

Fluorescence (Trp)-difference in emission max & intensity.

CD (far or near UV) - (2o or 3o) 

NMR 

DSC (calorimetry) 

Urea gradient gels - difference in the migrating rates between F and U.

Catalytic activity 

Chromophoric or fluorophoric probes/FRET 

TLS Online TPP Program

#Id: 1515

#Unit 2. Cellular Organization

Doublet contains one complete 13-protofilament microtubule (A tubule) and one additional tubules (B tubule) consisting of 10 protofilaments.

 

TLS Online TPP Program

#Id: 1516

#Unit 2. Cellular Organization

Triplet contains one complete 13-protofilament microtubule (A tubule) and two additional tubules (B & C tubule) consisting of 10 protofilaments each.

TLS Online TPP Program

#Id: 1517

#Unit 2. Cellular Organization

All microtubules are nucleated from structures known as microtubule-organizing centers, or MTOCs.

 

TLS Online TPP Program

#Id: 1518

#Unit 2. Cellular Organization

In MTOCs, 

The (−) end of the microtubule stays anchored in the MTOC while the (+) end extends away from it.

TLS Online TPP Program

#Id: 1519

#Unit 2. Cellular Organization

In cilia and flagella,

microtubules are assembled from an MTOC called a basal body.

TLS Online TPP Program

#Id: 1520

#Unit 2. Cellular Organization

Two different types of microtubule structures can exist, 

one in the T form bound to GTP 

one in the D form bound to GD