Techniques for Measuring Stability

Absorbance (e.g. Trp, Tyr) 

Fluorescence (Trp)-difference in emission max & intensity.

CD (far or near UV) - (2o or 3o) 

NMR 

DSC (calorimetry) 

Urea gradient gels - difference in the migrating rates between F and U.

Catalytic activity 

Chromophoric or fluorophoric probes/FRET 

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#Unit 4. Cell Communication and Cell Signaling

B cell is type of lymphocyte.

B cell synthesized and maturation in Bone marrow.

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#Unit 4. Cell Communication and Cell Signaling

Stages in hematopoiesis

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#Unit 4. Cell Communication and Cell Signaling

B cell properties

1.    They are APCs

2.    They can express MHC2

3.    Have surface marker  CD19

4.    Antibodies at surface of b cell – IgM, IgD

5.    Part of Adaptive immunity

6.    B cell convert into plasma cell

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#Unit 4. Cell Communication and Cell Signaling

Surface protein on B cell

CR2(CR21)

CD19

TAPA-1(CD81)

 mIg

Igα/ Igβ

(CD79a and CD79B)

 TLR

CD40

B7 (CD80/86)

ICAM-1

MHC-1 and 2

CD 22

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#Unit 4. Cell Communication and Cell Signaling

Formation of mature B-cell

TLS Online TPP Program

#Id: 1907

#Unit 4. Cell Communication and Cell Signaling

Progress through their developmental program