This combination of two positive feedback loops:

one that activates a CDK1 activator (Cdc25C)

one that inhibits a CDK1 inhibitor (Wee1)

results in the abrupt and irreversible transition from G2 into mitosis.

TLS Online TPP Program

#Id: 8541

#Unit 1. Structure and Function of Biomolecules

The dependent variable is the rate of the reaction. 

The independent variables are the concentration of enzyme, the concentration of the substrate, the temperature, and the addition of an inhibitor

TLS Online TPP Program

#Id: 8542

#Unit 1. Structure and Function of Biomolecules

If the concentration of the enzyme or substrate is increased, the rate of the reaction will be increased as well. 

If the temperature of the enzymes surrounding environment is increased, then the rate of the reaction will also be increased. 

If the substrate concentration is increased then the rate of the reaction will increase. 

If an inhibitor is added then the rate of the reaction will be decreased significantly

TLS Online TPP Program

#Id: 8543

#Unit 1. Structure and Function of Biomolecules

One drawback from the Eadie–Hofstee approach is that neither ordinate nor abscissa represent independent variables: both are dependent on reaction rate. Thus any experimental error will be present in both axes.

TLS Online TPP Program

#Id: 8544

#Unit 1. Structure and Function of Biomolecules

The abscissa refers to the (x) coordinate and the ordinate refers to the (y) coordinate of a standard two-dimensional graph.

TLS Online TPP Program

#Id: 8545

#Unit 1. Structure and Function of Biomolecules

Irreversible inhibitors, such as the organophosphorus and organomercury compounds, cyanide, carbon monoxide and hydrogen sulphide, combine with the enzyme to form a covalent bond. 

TLS Online TPP Program

#Id: 8546

#Unit 1. Structure and Function of Biomolecules

The extent of irreversible inhibition of the enzyme is dependent upon the reaction rate constant (and hence time) for covalent bond formation and upon the amount of inhibitor present.