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TLS Online TPP Program

#Id: 8912

When all the kinetochores have properly attached to spindle microtubules, APC/CCdc20 directs ubiquitinylation of securin and mitotic cyclins

#Unit 2. Cellular Organization #CELL CYCLE #Part B Pointers
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TLS Online TPP Program

#Id: 866

#Unit 13. Methods in Biology

The standard PCR does not efficiently amplify sequences much longer than about 3 kb.
TLS Online TPP Program

#Id: 867

#Unit 13. Methods in Biology

Size of the primer negatively affects the efficiency of PCR.
TLS Online TPP Program

#Id: 868

#Unit 13. Methods in Biology

PCR amplification is mathematically counted as 2n So 10 cycle leads to 1024 times amplification
TLS Online TPP Program

#Id: 869

#Unit 13. Methods in Biology

The efficiency of PCR can be calculated by using Y = X (1+ E)n X is number of templates, E is mean PCR efficiency, n is number is PCR cycle. 


 
TLS Online TPP Program

#Id: 870

#Unit 13. Methods in Biology

Variants of PCR has been devised for 
a. Efficiency
b. Sequence specific amplification
c. Mutation analysis
TLS Online TPP Program

#Id: 871

#Unit 13. Methods in Biology

Variants of PCR has been devised to enhance efficiency are 
a. qPCR with SYBR, Taqman and MB 
b. Nested PCR
c. Touchdown PCR
d. Hot-start PCR