Nurturing Life Sciences
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#Unit 13. Methods in Biology
Methylation interference assay Dimethyl sulphate (DMS) methylates purines on DNA strand. Methylate DNA prior to adding protein. Protein binding is prevented at methylated sites. After treatment and removal of protein of interest, treat with piperidine or NaOH which cuts strand at modified bases.
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#Unit 4. Cell Communication and Cell Signaling
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#Unit 11. Evolution and Behavior
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#Unit 1. Molecules and their Interaction Relevant to Biology
Potential energy of Dipole-Dipole Interaction
V is the potential energy
μ is the dipole moment
ϵo is the vacuum permittivity.
R is the length between the two nuclei
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#Unit 3. Fundamental Processes
DNA polymerase binds two divalent metal ions (typically Mg2+ orZn2+) that alter the chemical environment around the correctly base-paired dNTP and the 3’-OH of the primer