T4 DNA ligase Links 5-phosphate and 3-hydroxyl ends via phosphodiester bond.

TLS Online TPP Program

#Id: 2791

#Unit 13. Methods in Biology

notSelectivity or separation factor, a, which can also be viewed as simply the relative retention ratio for the two analytes:

TLS Online TPP Program

#Id: 2792

#Unit 13. Methods in Biology

Chromatography columns are considered to consist of a number of adjacent zones in each of which there is sufficient space for an analyte to completely equilibrate between the two phases, Each zone is called a theoretical plate (having no N).

TLS Online TPP Program

#Id: 2793

#Unit 13. Methods in Biology

The length of column containing one theoretical plate is referred to as the plate height, H, which has units of length normally in micrometers.

TLS Online TPP Program

#Id: 2794

#Unit 13. Methods in Biology

Plate height is simply related to the width of the analyte peak, expressed in terms of its standard deviation s , and the distance it travelled within the column, x. Specifically

TLS Online TPP Program

#Id: 2795

#Unit 13. Methods in Biology

The smaller the value of H and the greater the value of N, the more efficient is the column in separating a mixture of analytes.

TLS Online TPP Program

#Id: 2796

#Unit 13. Methods in Biology

The number of theoretical plates in the whole column of length L is equal to L divided by the plate height: