The more metabolically active cells that are present in the culture, the more formazan will be generated, and so this assay provides a readout of the number of live cells as a function of time.

TLS Online TPP Program

#Id: 716

#Unit 3. Fundamental Processes

The unit of DNA in which an individual act of replication occurs is called the replicon

TLS Online TPP Program

#Id: 717

#Unit 3. Fundamental Processes

“Replicon” is usually applied to eukaryotic chromosomes to describe a unit of replication that contains an origin; trans-acting regulator protein

TLS Online TPP Program

#Id: 718

#Unit 3. Fundamental Processes

A replicon has origin at which replication is initiated. It may also have a terminus at which replication stops.

TLS Online TPP Program

#Id: 719

#Unit 3. Fundamental Processes

A genome in a bacterial cell has a single replication origin and thus constitutes a single replicon.

TLS Online TPP Program

#Id: 720

#Unit 3. Fundamental Processes

Each haploid bacterium typically has a single chromosome, so this type of replication control is called single copy.

TLS Online TPP Program

#Id: 721

#Unit 3. Fundamental Processes

The single circular chromosomes of Sulfolobus species have three origins and thus are composed of three replicons.