Nurturing Life Sciences
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#Unit 13. Methods in Biology
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Propidium iodide-labeled cells can be identify cells within the populations are at each stage of the cell cycle.
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Propidium iodide-labeled cells in order asApoptotic cellsG1/G0SG2/M
Propidium iodide-labeled cells in order as
Apoptotic cells
G1/G0
S
G2/M
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Other dyes that bind to DNA and allow for similar types of cell cycle analysis include DAPI, Hoechst 33342, and 7-aminoactinomycin D (AAD).
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Carboxyfluorescein succinimidyl ester (CFSE) is more correctly named carboxyfluorescein diacetate succinimidyl ester (CFDASE) very commonly used to detect state of cell
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(CFSE) or carboxyfluorescein diacetate succinimidyl ester (CFDASE) is nontoxic to cells hence retained till many cycles