(CFSE) or carboxyfluorescein diacetate succinimidyl ester (CFDASE) is nontoxic to cells hence retained till many cycles

TLS Online TPP Program

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#Unit 3. Fundamental Processes

There are two basic types of DNA synthesis

TLS Online TPP Program

#Id: 5883

#Unit 3. Fundamental Processes

Semiconservative replication

DNA repair reaction

TLS Online TPP Program

#Id: 5884

#Unit 3. Fundamental Processes

All prokaryotic and eukaryotic DNA polymerases share the same fundamental type of synthetic activity -an antiparallel synthesis from 5’ to 3’ from a template that is 3’ to 5’. This means adding nucleotides one at a time to a 3’–OH end.

TLS Online TPP Program

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#Unit 3. Fundamental Processes

Hydrolysis of pyrophosphate is the driving force for DNA synthesis

TLS Online TPP Program

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#Unit 3. Fundamental Processes

The DNA polymerase monitors the ability of the incoming nucleotide to form an A:T or G:C base pair, rather than detecting the exact nucleotide that enters the active site in a DNA polymerase with significantly reduced discrimination

between dNTPs and rNTPs.

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#Unit 3. Fundamental Processes

Only when a correct base pair is formed are the 3’-OH of the primer and the a-phosphate of the incoming nucleoside triphosphate in the optimum position for catalysis to occur.