Nurturing Life Sciences
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#Unit 3. Fundamental Processes
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How tightly does core enzyme v. holoenzyme bind DNA?
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The effect of temperature on the dissociation of the polymerase–T7 DNA complex.
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higher temperature favors tighter binding between RNA polymerase holoenzyme and T7 DNA
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Isomerization, does not require energy derived from ATP hydrolysis and is instead the result of a spontaneous conformational change in the DNA– enzyme complex to a more energetically favorable form.