CFSE labeling amount of fluorescence emitted is cut in half each time the cell divides.

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#Unit 1. Molecules and their Interaction Relevant to Biology

Under these conditions, the reactions have an identical dissociation constant Ki , again called the inhibitor constant. 

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#Unit 1. Molecules and their Interaction Relevant to Biology

In the presence of a non-competitive inhibitor which will bind equally well to E or to ES- Vmax decreases but not Km 

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#Unit 1. Molecules and their Interaction Relevant to Biology

In case of nonidentical dissociation constants, MIXED TYPE INHIBITION TAKES PLACE 

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#Unit 1. Molecules and their Interaction Relevant to Biology

In the situation where Ki > K1 , This situation has been termed competitive-noncompetitive inhibition

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#Unit 1. Molecules and their Interaction Relevant to Biology

In the situation where Ki< K1, This form of mixed inhibition has been termed noncompetitive- uncompetitive inhibition

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#Unit 1. Molecules and their Interaction Relevant to Biology