Overall, there are three pillars electrophoresis

Current / voltage

Medium - (buffer/liquid) - with a precise pH value and a constant ionic strength.

 Analytes - A compound, which is to be detected or assayed in a given sample.

Electrophoretic resolution mainly can be change by manipulating current and buffer system.

The higher the buffer concentration, the more electric power is applied, resulting in higher heat (joule effect) development.

Smiling Effect, Venetian Blind Effect & Electroendosmosis reduces the efficiency of electrophoresis.

TLS Online TPP Program

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#Unit 13. Methods in Biology

Molecular beacons are FRET-labelled single-stranded hairpin-shaped oligonucleotide probes originally developed by Tyagi and Kramer in 1996.

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#Unit 1. Molecules and their Interaction Relevant to Biology

poly(Pro) helix are stabilized by water molecule hydrogen bonding

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#Unit 3. Fundamental Processes

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#Unit 4. Cell Communication and Cell Signaling

B cells can be negatively signaled through CD22 and FcγRIIb a receptor that recognizes the presence of IgG containing immune complexes in the blood.

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#Id: 9607

#Unit 4. Cell Communication and Cell Signaling

In resting state,  the activation loop is not phosphorylated and assumes a conformation that blocks kinase activity.

In insulin receptor, it prevents binding of ATP. 

In FGF receptor, it prevents binding of substrate.