Overall, there are three pillars electrophoresis

Current / voltage

Medium - (buffer/liquid) - with a precise pH value and a constant ionic strength.

 Analytes - A compound, which is to be detected or assayed in a given sample.

Electrophoretic resolution mainly can be change by manipulating current and buffer system.

The higher the buffer concentration, the more electric power is applied, resulting in higher heat (joule effect) development.

Smiling Effect, Venetian Blind Effect & Electroendosmosis reduces the efficiency of electrophoresis.

TLS Online TPP Program

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#Unit 1. Molecules and their Interaction Relevant to Biology

TLS Online TPP Program

#Id: 4857

#Unit 1. Molecules and their Interaction Relevant to Biology

TLS Online TPP Program

#Id: 4860

#Unit 1. Molecules and their Interaction Relevant to Biology

Hanes plot 

TLS Online TPP Program

#Id: 4862

#Unit 1. Molecules and their Interaction Relevant to Biology

The Eadie-Hofstee plot

     

TLS Online TPP Program

#Id: 4914

#Unit 1. Molecules and their Interaction Relevant to Biology

TLS Online TPP Program

#Id: 4916

#Unit 1. Molecules and their Interaction Relevant to Biology

Lineweaver-Burk plot showing the effect of competitive inhibition; (b) the same, showing plots for several inhibitor concentrations at fixed enzyme concentrations.