Nurturing Life Sciences
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#Unit 3. Fundamental Processes
Initiation of replication in E.Coli at oriC starts with formation of a complex that ultimately requires six proteins: DnaA, DnaB, DnaC, HU, gyrase, and SSB
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The minimal origin is defined by the distance between the outside members of the 13-mer and 9-mer repeats.
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The four 9-bp consensus sequences on the right side of oriC provide the initial binding sites for DnaA-ATP
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A two-state assembly model explains melting of 13mer during initiation
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DNA strand synthesis requires the four deoxynucleoside triphosphates—dGTP, dCTP, dATP, and dTTP in equal in vivo concentration