TLS Online TPP Program

#Id: 2626

#Unit 13. Methods in Biology

Steps of nick translation- 

Low concentrations of DNase I are used to make occasional single-strand nicks in the double-stranded DNA that is intended as the gene probe.

DNA polymerase then fills in the nicks, using an appropriate dNTP, at the same time making a new nick to the 3′ side of the previous one.

TLS Online TPP Program

#Id: 2627

#Unit 13. Methods in Biology

Types and examples of typical enzymes used in the manipulation of nucleic acids

TLS Online TPP Program

#Id: 2628

#Unit 13. Methods in Biology

Types and examples of typical enzymes used in the manipulation of nucleic acids

TLS Online TPP Program

#Id: 2629

#Unit 13. Methods in Biology

End-labelling of a gene probe at the 5′ end with alkaline phosphatase and polynucleotide kinase. 

TLS Online TPP Program

#Id: 2630

#Unit 13. Methods in Biology

End-labelling of a gene probe at the 3′ end using terminal transferase. Note that the addition of a labeled dNTP at the 3′ end alters the sequence of the gene probe.

TLS Online TPP Program

#Id: 2631

#Unit 13. Methods in Biology

S1 mapping is used to locate the 5’- or 3’-ends of RNAs and to quantify the amount of a given RNA in cells at a given time.