Nurturing Life Sciences
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#Unit 13. Methods in Biology
Phase contrast and Differential interference microscopy are used to image live cells and unstained tissues.
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Unobstructed direct light passes through a region of the phase plate that both transmits only a small percentage of the light and changes its phase slightly
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Phase-contrast microscopy is suitable for observing single cells or thin cell layers.
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DIC microscopy is used for visualizing extremely small details and thick objects.