TLS Online TPP Program

#Id: 2999

#Unit 13. Methods in Biology

Flow chart of the fractionation protocol of these membranes from tissue homogenates
using differential centrifugation and density gradient methodology.

TLS Online TPP Program

#Id: 3000

#Unit 13. Methods in Biology

Crude differential centrifugation techniques can be conveniently employed to isolate
intact mitochondria and microsomes.

TLS Online TPP Program

#Id: 3001

#Unit 13. Methods in Biology

Caesium chloride is widely used for the banding of DNA and the isolation of
plasmids, nucleoproteins and viruses

TLS Online TPP Program

#Id: 3002

#Unit 13. Methods in Biology

Sodium bromide and sodium iodide are employed for the fractionation of lipoproteins
and the banding of DNA or RNA molecules, respectively.

TLS Online TPP Program

#Id: 3003

#Unit 13. Methods in Biology

For the separation of membrane vesicles derived from tissue homogenates, ultra-pure
DNase-, RNase and protease-free sucrose represents a suitable and widely employed
medium for the preparation of stable gradients.

TLS Online TPP Program

#Id: 3004

#Unit 13. Methods in Biology

For isopycnic separation , density centrifugation is continued until the buoyant density
of the particle of interest and the density of the gradient are equal.