TLS Online TPP Program

#Id: 4610

#Unit 13. Methods in Biology

Normal cells treated with sodium chromate allowed to absorb and induced to death signals as T-CTLs Death of the target cells is indicated by the release of 51Cr into the supernatant

TLS Online TPP Program

#Id: 4611

#Unit 13. Methods in Biology

FITC binds to Annexin V to phosphatidyl serine which are found on apoptotic cells, analyzed by FACS

TLS Online TPP Program

#Id: 4612

#Unit 13. Methods in Biology

The TUNEL Assay measures Apoptotically Generated DNA Fragmentation.

TLS Online TPP Program

#Id: 4613

#Unit 13. Methods in Biology

TUNEL method uses TdT to add BrdU to fixed and permeabilized cells which can only incorporated into fragmented DNA of apoptotic cells.

TLS Online TPP Program

#Id: 4614

#Unit 13. Methods in Biology

BrdU is incorporated into the newly synthesized DNA, and is then detected with fluorescently labeled anti-BrdU antibodies

TLS Online TPP Program

#Id: 4615

#Unit 13. Methods in Biology

Caspases western blotting assay is also used to detect apoptosis