Nurturing Life Sciences
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#Unit 13. Methods in Biology
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Cryoelectron microscopy allows examination of hydrated, unfixed, and unstained specimens in the TEM by maintaining them at -196oC (liquid nitrogen).
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This method has been used to generate models of ribosomes, the muscle calcium pump, and 3-D structure of a poliovirus capsid.
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The smaller the value of D, the better the resolution. The value of D is given by the equation.
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Resolving power or resolution is the ability to distinguish between two very closely positioned objects.