Nurturing Life Sciences
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#Unit 13. Methods in Biology
The unit of s is seconds Most values of s are between 10-13 and 10-11 second. Toeliminate the exponents, the value of s is usually reported in svedbergs (S). 1 svedberg 10-13 second.
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During differential sedimentation the largest class of particles forms a pellet on thebottom of the centrifuge tube, leaving smaller-sized structures within the supernatant.
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Isopycnic separation, centrifugation is continued until the desired particles havereached their isopycnic position in the liquid density gradient.
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Flow chart of the fractionation protocol of these membranes from tissue homogenatesusing differential centrifugation and density gradient methodology.
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