Viruses targets endocytic recycling pathway for cell‐to‐cell spread

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#Unit 13. Methods in Biology

If the antibody preparation is polyclonal and has a range of affinities, Scatchard plot yields a curved line whose slope is constantly changing. The average affinity constant K0 can be calculated by determining the value of Ka when half of the binding sites are occupied (i.e., when r  1 in this example). In this graph, antiserum #3 has a higher affinity (K0  2.4  108) than antiserum #4 (K0  1.25  108)

#Id: 4669

#Unit 13. Methods in Biology

The bacterium Streptococcus pyogenes, for example, expresses cell-wall proteins called M antigens. Antibodies produced to streptococcal M antigens have been shown to cross-react with several myocardial and skeletal muscle proteins and have been implicated in heart and kidney damage following streptococcal infections. 

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#Unit 13. Methods in Biology

Glycosphingolipids as determinants of blood groups

#Id: 4671

#Unit 13. Methods in Biology

#Id: 4672

#Unit 13. Methods in Biology

A precipitation curve for a system of one antigen and its antibodies. This plot of the amount of antibody precipitated versus increasing antigen concentrations (at constant total antibody) reveals three zones: 

#Id: 4673

#Unit 13. Methods in Biology