Nurturing Life Sciences
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Bergmann’s rule: Populations in colder climates tend to have greater body mass than those in warmer climates.
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#Unit 13. Methods in Biology
Molecular beacons gives an idea for annealing.
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TaqMan assay gives an idea for alleles in Real time PCR.
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TaqMan assay also applicable in analyzing variations in the allele or deletion of base substitution.
TaqMan assay also applicable in analyzing variations in the allele or deletion
of base substitution.
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Touch down PCR are derived to enhance primer binding in the annealing step.
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A modification of overlap extension PCR may also be used to construct directed mutations; this is termed megaprimer PCR .
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Long primers forms most probably dimers, so taq pol amplifying this (in low temp) also.