Downstream of Ras is a highly conserved cascade of three protein kinases-

Ras → Raf → MEK → MAP kinase

TLS Online TPP Program

#Id: 865

#Unit 13. Methods in Biology

The size of a PCR product is mainly determined by the binding location of the PCR primers.

TLS Online TPP Program

#Id: 866

#Unit 13. Methods in Biology

The standard PCR does not efficiently amplify sequences much longer than about 3 kb.

TLS Online TPP Program

#Id: 867

#Unit 13. Methods in Biology

Size of the primer negatively affects the efficiency of PCR.

TLS Online TPP Program

#Id: 868

#Unit 13. Methods in Biology

PCR amplification is mathematically counted as 2n So 10 cycle leads to 1024 times amplification

TLS Online TPP Program

#Id: 869

#Unit 13. Methods in Biology

The efficiency of PCR can be calculated by using Y = X (1+ E)n X is number of templates, E is mean PCR efficiency, n is number is PCR cycle. 

 

TLS Online TPP Program

#Id: 870

#Unit 13. Methods in Biology

Variants of PCR has been devised for 

a. Efficiency

b. Sequence specific amplification

c. Mutation analysis