Movement of the GSVs to the plasma membrane is regulated by two monomeric GTP-binding proteins -RalA and Rab10

TLS Online TPP Program

#Id: 6892

#Unit 3. Fundamental Processes

To modify DNA at a new position requires the action of a de novo methyltransferase, which recognizes DNA by virtue of a specific sequence. It acts only on unmethylated DNA to add a methyl group to one strand.

TLS Online TPP Program

#Id: 2595

#Unit 13. Methods in Biology

Oligonucleotide-directed mutagenesis

The basic PCR mutagenesis system involves the use of two primary PCR reactions to produce two overlapping DNA fragments, both bearing the same mutation in the overlap region; this technique is thus termed overlap extension PCR .

TLS Online TPP Program

#Id: 6395

#Unit 2. Cellular Organization

Major enzyme is Tryptophan Synthase whose activity is reduced when trp concentration is high

TLS Online TPP Program

#Id: 6254

#Unit 8. Inheritance Biology

Complementation test 

Determining whether two mutations associated with a specific phenotype represent two different forms of the same gene (alleles) or are variations of two different genes.

TLS Online TPP Program

#Id: 1577

#Unit 4. Cell Communication and Cell Signaling

β-catenin binds to TCF to displace the Gro repressor and recruits co-activator proteins Pygo, LGS, to activate target gene expression.