PTI and fungal pathogen entry into epidermal tissue induces several specific cytochrome P450 enzymes, and this leads to accumulation of the indole glucosinolate 4‐methoxyindol‐3‐ylmethylglucosinolate (4M13G).

TLS Online TPP Program

#Id: 2416

#Unit 3. Fundamental Processes

Model of Intrinsic Termination

TLS Online TPP Program

#Id: 2417

#Unit 3. Fundamental Processes

Termination requires that all hydrogen bonds holding the RNA–DNA hybrid together must be broken, after which the DNA duplex reforms.

TLS Online TPP Program

#Id: 2418

#Unit 3. Fundamental Processes

he ability of the enzyme to terminate in vitro, however, is strongly influenced by parameters such as the ionic strength and temperature at which the reaction is performed.

TLS Online TPP Program

#Id: 2419

#Unit 3. Fundamental Processes

Termination depends on the RNA product and is not determined simply by scrutiny of the DNA sequence during transcription.

TLS Online TPP Program

#Id: 2420

#Unit 3. Fundamental Processes

Intrinsic terminators—that is, those that do not require auxiliary rho factor (r), as described shortly—require a GC–rich hairpin to form in the secondary structure of the RNA being transcribed.

TLS Online TPP Program

#Id: 2421

#Unit 3. Fundamental Processes

Rho-dependent terminators are defined by the need for addition of rho factor in vitro, and mutations show that the factor is involved in termination in vivo.