TLS Online TPP Program

#Question id: 1240


Pull-down assays using proteins that ONLY bind to the GTP-bound version of the small G-protein can be used to detect when small G proteins like Rac1 have been activated by various growth factors.  You were given two lysates, one having been treated with platelet-derived growth factor (PDGF) and the other not treated with PDGF. This method is similar to performing co-IPs, so based on that knowledge, which of the following steps is erroneous?

#Unit 4. Cell Communication and Cell Signaling
  1. To assay for levels of active Rac1, PAK1-beads (the protein that only binds GTP-bound Rac1-conjugated to beads similar to Nickel beads) are added to each of the lysates described above and incubated on a rocking platform (on ice). 

  2. The tubes are vortexed well, loading buffer is added, and the samples are heated to aid in protein denaturation.

  3. The samples are loaded and analyzed by SDS-polyacrylamide gel electrophoresis, then the proteins are transferred to a membrane. 

  4. The membrane (a.k.a. blot) will be probed with the anti-Rac antibody.

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TLS Online TPP Program

#Question id: 10719

#Unit 10. Ecological Principles

Five Hypotheses Explain How Species Richness Affects Community Services, Following statement are given
A) Community services decline with decreased species richness in a stepwise fashion.
B) Species that play the same roles in a community can compensate for each other if some are lost under particular conditions
C) Species richness declines even slightly from its natural levels, community services are reduced immediately
D) Community services change as the number of species increases or decreases, but that the direction of change is not predictable.

TLS Online TPP Program

#Question id: 23118

#Unit 2. Cellular Organization

How many bp distance require to Two points helix may actually be one on another on nucleosome surface.

TLS Online TPP Program

#Question id: 2636

#Unit 1. Molecules and their Interaction Relevant to Biology

Which of the following events occur when E. coli is starved for tryptophan?

I.  The ribosome translates leader peptide completely

II. Transcription reads through attenuator into trp EDCBA

III. Tryptophanyl tRNA is not formed

IV. A stem loop structure involving regions 3 and 4 is formed at attenuator site

TLS Online TPP Program

#Question id: 19069

#Unit 4. Cell Communication and Cell Signaling

Pathogenesis included except__

TLS Online TPP Program

#Question id: 753

#Unit 1. Molecules and their Interaction Relevant to Biology

The renaturation of regions of complementarity between different nucleic acid strands is known as___________