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TLS Online TPP Program
#Question id: 18605
#Unit 13. Methods in Biology
Thermal modulation are used in PCR for the functions of
TLS Online TPP Program
#Question id: 18606
#Unit 13. Methods in Biology
In the PCR denaturation temperature calculated by using the
TLS Online TPP Program
#Question id: 18607
#Unit 13. Methods in Biology
The PCR consists of three defined sets of times and temperatures, termed steps
a) Denaturation at 94°C, for 0.5 min
b) Primer annealing at 55°C for 1.5 min
c) Extension at 72°C for 1 min
d) only denaturation and extension
TLS Online TPP Program
#Question id: 18608
#Unit 13. Methods in Biology
In an experiment, A 5kb DNA undergoes PCR using primer, after electrophoresis instead getting a 5kb band, two set of PCR band are found of 5kb and 3kb, this is due to
TLS Online TPP Program
#Question id: 18609
#Unit 13. Methods in Biology
An experiment performed using cell for replication and test tube for PCR by using two conditions of radio labelling
1) by radio labeling primer
2) by radio labeling dNTP
the most possible result will be
TLS Online TPP Program
#Question id: 18610
#Unit 13. Methods in Biology
In an experiment, it was found that the value of Tm for DNA is 45 ̊C, when cell have 22% GC content at the above Tm, then what will be the effect on the value of Tm if GC% increases to 60%