You have isolated two mutations in the Lac operon that cause constitutive expression of Lac genes. You designate these mutants Lac1– and Lac2–. Making use of an F' that carries the Lac operon with the LacY gene mutated, you construct strains that you test for both ß-galactosidase activity and Lac permease activity with results shown below.

     

Classify each mutation as dominant or recessive and as cis- or trans-acting, giving the experimental result that allows you to arrive at each conclusion. Finally, deduce what type of Lac mutation best fits the properties of Lac 1– and of Lac 2–.

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#Question id: 18791

#Unit 13. Methods in Biology

Which of the following Assay is calorimetric assay to detect cell division calorimetric assay to detect cell division

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#Question id: 18792

#Unit 13. Methods in Biology

A yellow tetrazole is reduced by metabolically active cells to form insoluble, purple formazan dye crystals detected at 570nm. For this phenomenon which of the following compound should be uptake by cell

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#Question id: 18793

#Unit 13. Methods in Biology

Which of the following Assay can measure cell proliferation or cell death or active cells

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#Question id: 18794

#Unit 13. Methods in Biology

Which of the following compound is rapidly phosphorylated and incorporated in its place into newly synthesized DNA and is used to detect replicating cells.

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#Question id: 18795

#Unit 13. Methods in Biology

Which of the following compound can be used as analogue to Propidium Iodide

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#Question id: 18796

#Unit 13. Methods in Biology

Newly replicating cell can be selectively killed by photolysis incorporating