Nucleosome DNA wraps around the histone protein 1.65 times, so create a writhe of –1.65 and so DLk should be -1.65. But when the change in linking number associated with each nucleosome was measured, the number was lower than this, approximately –1.2 for each nucleosome added. This discrepancy is referred to as the 

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#Question id: 27374

#Unit 13. Methods in Biology

An aqueous solution contains two compounds X and Y. This solution gave absorbance values of 1.0 and 0.4 at 220 and 280 nm, respectively, in a 1 cm path length cell. Molar absorption coefficients (ε) of the compounds X and Y are as shown in the table below.

The concentration of Y in the solution is___________ mM.

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#Question id: 27477

#Unit 13. Methods in Biology

The length of a double helical DNA molecule is 13.6 km. If the DNA double helix weighs 1 ×10−18 g per 1000 nucleotide pairs and rise per base pair is 3.4 Å, then weight of the double helical DNA molecule (in nanogram) will be ______ (in integer).

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#Question id: 28162

#Unit 13. Methods in Biology

Which of the following method for the purification of plant DNA, which forms an insoluble complex with nucleic acids.

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#Question id: 28163

#Unit 13. Methods in Biology

Which of the following is component belong to LB medium involves in  Growing and harvesting a bacterial culture

a. Tryptone

b. Yeast extract

c. NaCI

d. Glucose

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#Question id: 28164

#Unit 13. Methods in Biology

It is possible to calculate the number of clones that must be present in a gene library to give a probability of obtaining a particular DNA sequence. This correct formula is:

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#Question id: 28165

#Unit 13. Methods in Biology

Q. Arrange the steps of cloning and selection in YAC vector

a. BamHI digestion creates linear chromosome with telomeric ends.

b. Transformation

c. EcoRI digestion creates two arms, each with a selectable marker (X or Y).

d. Selection on the basis of marker