TLS Online TPP Program

#Question id: 15844


You have isolated two temperature-sensitive mutations in phage l that you suspect may be in the same gene. These phage mutants are called ts-1 and ts-2. Each mutant will form plaques at 35˚C but not at 42˚C. You cross ts-1 to ts-2 phage by coinfecting E. coli at the permissive temperature of 35˚C. When the resulting phage lysate is plated at 35˚C you count 10,000 plaques per ml of phage lysate, but when the same phage lysate is plated at 42˚C, there are only 80 plaques per ml.  
If the total size of the phage is 5 x 104 bp and the total genetic map length of the phage is 100 m.u., about how far apart are the mutations in base pairs?

#Unit 8. Inheritance Biology
  1. 500 bp
  2. 300 bp
  3. 800 bp
  4. 400 bp