You have isolated two temperature-sensitive mutations in phage l that you suspect may be in the same gene. These phage mutants are called ts-1 and ts-2. Each mutant will form plaques at 35˚C but not at 42˚C. You cross ts^-1 to ts^-2 phage by coinfecting E. coli at the permissive temperature of 35˚C. When the resulting phage lysate is plated at 35˚C you count 10,000 plaques per ml of phage lysate, but when the same phage lysate is plated at 42˚C, there are only 80 plaques per ml.  

If the total size of the phage is 5 x 10⁴ bp and the total genetic map length of the phage is 100 m.u., about how far apart are the mutations in base pairs?

#Question id: 2237

#Unit 2. Cellular Organization

Glucose enters erythrocytes via a GLUT-1 uniporter. As the levels of glucose in the bloodstream decrease between meals, what happens to the glucose in the cells?

#Question id: 2238

#Unit 2. Cellular Organization

Consider the transport of glucose into an erythrocyte by facilitated diffusion.  When the glucose concentrations are 5 mM on the outside and 0.1 mM on the inside, the free-energy change for glucose uptake into the cell is:  (These values may be of use to you:  R = 8.315 J/mol·K; T = 298 K; 9 (Faraday constant) = 96,480 J/V; N = 6.022×10²³ /mol.)

#Question id: 2239

#Unit 2. Cellular Organization

Consider the transport of K+ from the blood (where its concentration is about 4 mM) into an erythrocyte that contains 150 mM K+.  The transmembrane potential is about 60 mV, inside negative relative to outside.  The free-energy change for this transport process is:  (These values may be of use to you:  R = 8.315 J/mol.K; T = 298 K; 9 (Faraday constant) = 96,480 J/V; N = 6.022×10²³ /mol.)

#Question id: 2240

#Unit 2. Cellular Organization

 In which of the following cases is energy NOT needed for transmembrane transport?

a. Lysine moves into the cell against its concentration gradient via the Na+/lysine symporter.

b. Potassium ions (K+) move out of the cell down the K+ concentration gradient via potassium channels.

c. Glucose moves into the cell down its concentration gradient via a glucose uniporter.

d. Sodium ions (Na+) move out of the cell down the Na+ concentration gradient via sodium channels.

Which of the following are correct?

#Question id: 2241

#Unit 2. Cellular Organization

Which of the following statement(s) is (are) true of V-class proton pumps?

#Question id: 2242

#Unit 2. Cellular Organization

What two features of the structure of cholesterol make it somewhat amphipathic?