You have isolated two temperature-sensitive mutations in phage l that you suspect may be in the same gene. These phage mutants are called ts-1 and ts-2. Each mutant will form plaques at 35˚C but not at 42˚C. You cross ts-1 to ts-2 phage by coinfecting E. coli at the permissive temperature of 35˚C. When the resulting phage lysate is plated at 35˚C you count 10,000 plaques per ml of phage lysate, but when the same phage lysate is plated at 42˚C, there are only 80 plaques per ml. the relative order is sm→ts-2→ts-1, find out the distances you can calculate in m.u.?

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#Question id: 3656

#Unit 3. Fundamental Processes

In nucleotide excision repair, an endonuclease makes nicks on either side of the lesion.

TLS Online TPP Program

#Question id: 3657

#Unit 3. Fundamental Processes

Mutations in excision repair genes or a translesion DNA polymerase cause:

TLS Online TPP Program

#Question id: 3658

#Unit 3. Fundamental Processes

In E. coli, the UvrABC endonuclease removes _____________and other bulky lesions by recognizing the distortion these produce in the double helix.

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#Question id: 3659

#Unit 3. Fundamental Processes

In eukaryotes, gap filling in BER involves predominantly DNA polymerase β whereas the longer gaps generated in NER are filled by:

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#Question id: 3660

#Unit 3. Fundamental Processes

Which of the following is true for exchange of homologous regions between two DNA molecules?

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#Question id: 3661

#Unit 3. Fundamental Processes

______________ short fragment of DNA produced during replication of the lagging strand.