TLS Online TPP Program

#Question id: 15958


During the protoplast fusion which technique is most commonly used as it induces reproducible high frequency fusion accompanied with low toxicity to most cell types?

#Unit 12. Applied Biology
  1. Electrofusion
  2. High pH-high Ca2+ treatment
  3. Polyethylene glycol (PEG)
  4. Electroporator
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TLS Online TPP Program

#Question id: 2252

#Unit 2. Cellular Organization

The generation and maintenance of a membrane electric potential requires which of the following:

a. active pumping of ions       

b. ion-specific membrane channel proteins

c. ATP                                    

d. a generally permeable membrane

TLS Online TPP Program

#Question id: 2253

#Unit 2. Cellular Organization

Match the following lipid linked proteins (column I) with their function (Column II).

Column I

Column II

A. prenylated proteins

i. These proteins are located in a number of subcellular compartments, including the cytosol, the endoplasmic reticulum, the inner face of the plasma membrane, and the nucleus.

B. fatty acylated proteins

ii. The core structure of these proteins consists of phosphatidylinositol glycosidically linked to a linear tetrasaccharide composed of three mannose residues and one glucosaminyl residue.

C. glycosylphosphatidylinositol-linked proteins

iii. The proteins that have covalently attached lipids that are built from isoprene units

TLS Online TPP Program

#Question id: 2254

#Unit 2. Cellular Organization

If the intracellular [Ca2+] is 10−7 mol/L and the extracellular [Ca2+] is 2 × 10−3 mol/L, at what potential difference across the cell membrane will Ca2+ be at electrochemical equilibrium?

TLS Online TPP Program

#Question id: 2255

#Unit 2. Cellular Organization

Following statements are regarding to the Na+/K+ ATPase maintains the intracellular Na+ and K+ concentrations in animal cells. Which of the following statement is incorrect?

TLS Online TPP Program

#Question id: 2256

#Unit 2. Cellular Organization

Match the following inhibitors (Column I) with their effect (Column II).

Column I

Column II

A. A23187

I. endoplasmic reticulum Ca2+ ATPase inhibitor

B. valinomycin

II. Na+/K+ ATPase inhibitor

C. thapsigargin

III. K+ ionophore

D. ouabain

IV. Ca2+ ionophore

TLS Online TPP Program

#Question id: 2257

#Unit 2. Cellular Organization

Match the following property (Column I) with the transport mechanism (Column II).

Column I

Column II

A. Facilitated Transport

i. Requires specific protein

B. Active Transport

ii. Solute transported against its gradient

C. Cotransport

iii. Coupled to ATP hydrolysis

iv. Driven by movement of a cotransported ion down its gradient