TLS Online TPP Program

#Question id: 16137


You obtain 6 BACs (of known order, as shown below) and 7 STSs (of unknown order) that derive from a region of mouse chromosome 16 whose genomic sequence has not yet been finished.   
 
By PCR (using 20-bp primers at either end of each STS), you test each of the 6 BACs for the presence (+) or absence (-) of each of the 7 STSs. You obtain the following results:
 
How would you use the sequence information presented in to design two new STSs (with new PCR primer pairs) to replace STS5? (Call the new ones STS51 and STS52. STS51 should be present (+) in BAC B, and STS52 should be present (+) in BAC F.)

#Unit 13. Methods in Biology
  1. Primers that include the homologous sequence in STS51 and STS52. These primers should specifically amplify only STS52  not  STS51.
  2. Primers that include the non-homologous sequence in STS51 and STS52. These primers should specifically amplify both simultaneously  STS51 and  STS52.
  3. Primers that include the homologous sequence in STS51 and STS52. These primers should specifically amplify only STS51  not  STS52.
  4. Primers that include the non-homologous sequence in STS51 and STS52. These primers should specifically amplify either STS51 or STS52.
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TLS Online TPP Program

#Question id: 27952

#Unit 1. Molecules and their Interaction Relevant to Biology

Which of the following given set of condition will favours gluconeogenesis

TLS Online TPP Program

#Question id: 27953

#Unit 1. Molecules and their Interaction Relevant to Biology

[F26BP] concentration are related to the glycolysis, and gluconeogenesis

a.↑[F26BP] Stimulates glycolysis, and gluconeogenesis

b.↓ [F26BP] Inhibits glycolysis, and gluconeogenesis

c.↑ [F26BP] Stimulates glycolysis, inhibits gluconeogenesis

d↓ [F26BP] Inhibits glycolysis, stimulates gluconeogenesis

Select the condition of [F26BP] in which appropriate process will takes place 

TLS Online TPP Program

#Question id: 27954

#Unit 1. Molecules and their Interaction Relevant to Biology

Some of the statement is given regarding the Pyruvate kinase 
a. Pyruvate kinase is activated  by ATP, acetyl-CoA, long-chain fatty acids 
b. Pyruvate kinase is allosterically inhibited by ATP, acetyl-CoA, long-chain fatty acids
c. Accumulation of alanine allosterically inhibits pyruvate kinase M in muscles, slowing the production of pyruvate by glycolysis
d. Accumulation of alanine activate pyruvate kinase M in muscles, increases the production of pyruvate by glycolysis

Which of the following is incorrect

TLS Online TPP Program

#Question id: 27955

#Unit 1. Molecules and their Interaction Relevant to Biology

The liver PK isozyme (L form) is regulated by

TLS Online TPP Program

#Question id: 27956

#Unit 1. Molecules and their Interaction Relevant to Biology

Some of the enzyme differ in glycolysis and gluconeogenesis, Which of the following set of enzyme are different

TLS Online TPP Program

#Question id: 27957

#Unit 1. Molecules and their Interaction Relevant to Biology

Bicarbonate + Pyruvate→ Oxaloacetate

a. This reaction catalyzed by pyruvate carboxylase

b. This reaction catalyzed by carbonic anhydrases

c. Uses biotin as cofactor of pyruvate carboxylase

d. Uses biotin as cofactor ofby carbonic anhydrases

Which of the following is true about the given reaction