Following statements are regarding to enzymatic processing of benzo(a)pyrene to a more potent mutagen and carcinogen.

A. Liver enzymes, particularly P-450 enzymes, modify benzo(a)pyrene in a series of reactions, producing 7,8-diol-9,10-epoxide, a highly potent mutagenic species that reacts with DNA primarily at the N2 atom of a guanine base.

B. The adduct, (+)-trans-anti-B(a)P-N2-dG, causes polymerase to insert an A rather than a C opposite the modified G base. Next time the DNA is replicated, a T will be inserted opposite the A, and the mutation will be complete.

C. Potent mutagen majorly causes conversion of guanine (G) to thymine (T) bases, a transition mutation.

D. When this potent mutagen applied to cultured bronchial epithelial cells, activated benzo(a)pyrene induces many mutations, including activating mutations at codons 175, 248, and 273 of the p53 gene.

Which of the following statements are correct?

TLS Online TPP Program

#Question id: 18918

#Unit 13. Methods in Biology

Recognition Sequences for Some Type II Restriction Endonucleases 

Match them correctly

TLS Online TPP Program

#Question id: 18919

#Unit 13. Methods in Biology

What are REP1 and REP2 genes in the plasmid used for?

TLS Online TPP Program

#Question id: 18920

#Unit 13. Methods in Biology

Which selection system is used in a yeast plasmid recombinant?

TLS Online TPP Program

#Question id: 18921

#Unit 13. Methods in Biology

What is an auxotrophic mutant?

TLS Online TPP Program

#Question id: 18922

#Unit 13. Methods in Biology

For which of the following plasmids purification of a recombinant molecule from the transformed cell can be difficult?

TLS Online TPP Program

#Question id: 18923

#Unit 13. Methods in Biology

lacZ' gene present in a Puc series plasmid codes for which of the enzyme?