The various advantages of serum-free media could be

1. Improved reproducibility of results from different laboratories

2. Easier downstream processing of products from cultured cells

3. Biassays are free from interference due to serum proteins

4. They permit selective culture of diffrentiated cell types from the heterogeneous cultures

Which of the statements are true?

#Question id: 4461

#Unit 3. Fundamental Processes

According to some experiments, during initial transcription, the polymerase remains stationary on the promoter, unwinds downstream DNA, and pulls that DNA into itself, which model is this?

#Question id: 4462

#Unit 3. Fundamental Processes

Promoter escape is associated with the breaking of all interactions (at the given promoter) between;

#Question id: 4463

#Unit 3. Fundamental Processes

___?____ is a way to store and mobilize energy during transcription initiation, and its release upon escape is what enables polymerase to break free of the promoter and dislodge s factor from the core.

#Question id: 4464

#Unit 3. Fundamental Processes

Role of Gre factor;

#Question id: 4465

#Unit 3. Fundamental Processes

Main function of Nus proteins in transcription,

#Question id: 4466

#Unit 3. Fundamental Processes

As well as synthesizing the transcript, RNA polymerase performs two proofreading functions on that growing transcript;

i. Pyrophosphorolytic editing

ii. Hydrolytic editing

Choose correct processes accordingly,

A. The polymerase backtracks by one or more nucleotides and cleaves the RNA product, removing the error-containing sequence.

B. In this, the enzyme uses its active site, in a simple back-reaction,

C. Catalyze the removal of an incorrectly inserted ribonucleotide, by reincorporation of PPi

D. The enzyme can then incorporate another ribonucleotide in its place in the growing RNA chain.