At high salt concentrations, the mRNA containing poly(A) tails binds to the complementary oligo(dT) molecules of the affinity column, and so, mRNA will be retained; all other RNA molecules can be washed through the column by further high salt solution. finally, the bound mRNA can be eluted by using

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#Question id: 15842

#Unit 8. Inheritance Biology

In the yeast cross of ade2 met14 his3 x + + +, a total of 120 tetrads are analyzed. The markers ade2, met14, and his3 are abbreviated a, m, and h and the wild type allele of each gene is indicated by +.

      

Given three locus from above, which locus shows the tetratype (T)

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#Question id: 15843

#Unit 8. Inheritance Biology

You have isolated two temperature-sensitive mutations in phage l that you suspect may be in the same gene. These phage mutants are called ts-1 and ts-2. Each mutant will form plaques at 35˚C but not at 42˚C. You cross ts-1 to ts-2 phage by coinfecting E. coli at the permissive temperature of 35˚C. When the resulting phage lysate is plated at 35˚C you count 10,000 plaques per ml of phage lysate, but when the same phage lysate is plated at 42˚C, there are only 80 plaques per ml.           What is the distance between the ts-1 and ts-2 mutations in m.u.?

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#Question id: 15844

#Unit 8. Inheritance Biology

You have isolated two temperature-sensitive mutations in phage l that you suspect may be in the same gene. These phage mutants are called ts-1 and ts-2. Each mutant will form plaques at 35˚C but not at 42˚C. You cross ts^-1 to ts^-2 phage by coinfecting E. coli at the permissive temperature of 35˚C. When the resulting phage lysate is plated at 35˚C you count 10,000 plaques per ml of phage lysate, but when the same phage lysate is plated at 42˚C, there are only 80 plaques per ml.  

If the total size of the phage is 5 x 10⁴ bp and the total genetic map length of the phage is 100 m.u., about how far apart are the mutations in base pairs?

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#Question id: 15845

#Unit 8. Inheritance Biology

You have isolated two temperature-sensitive mutations in phage l that you suspect may be in the same gene. These phage mutants are called ts-1 and ts-2. Each mutant will form plaques at 35˚C but not at 42˚C. You cross ts-1 to ts-2 phage by coinfecting E. coli at the permissive temperature of 35˚C. When the resulting phage lysate is plated at 35˚C you count 10,000 plaques per ml of phage lysate, but when the same phage lysate is plated at 42˚C, there are only 80 plaques per ml. Find out the relative order of sm, ts-1 and ts-2?

TLS Online TPP Program

#Question id: 15846

#Unit 8. Inheritance Biology

You have isolated two temperature-sensitive mutations in phage l that you suspect may be in the same gene. These phage mutants are called ts-1 and ts-2. Each mutant will form plaques at 35˚C but not at 42˚C. You cross ts-1 to ts-2 phage by coinfecting E. coli at the permissive temperature of 35˚C. When the resulting phage lysate is plated at 35˚C you count 10,000 plaques per ml of phage lysate, but when the same phage lysate is plated at 42˚C, there are only 80 plaques per ml. the relative order is sm→ts-2→ts-1, find out the distances you can calculate in m.u.?

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#Question id: 15847

#Unit 4. Cell Communication and Cell Signaling

Pathogen-associated molecular patterns (or PAMPs) are common foreign structures that characterize whole groups of pathogens. It is these unique antigenic structures that the immune system frequently recognizes first. Animals, both invertebrates and vertebrates, have evolved to express several types of cell surface and soluble proteins that quickly recognize many of these PAMPs; a form of pathogen profiling. For example, encapsulated bacteria possess a polysaccharide coat with a unique chemical structure that is not found on other bacterial or human cells. White blood cells naturally express a variety of receptors, collectively referred to as pattern recognition receptors (PRRs), that specifically recognize these sugar residues, sugar residues is recognised by?