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                                                    #Question id: 18705
#Unit 13. Methods in Biology
What will weight in picogram in human genome 
 
                                                    TLS Online TPP Program
                                                    #Question id: 18706
#Unit 13. Methods in Biology
Sources of thermostable DNA polymerases with proofreading (3′–5′ exonuclease) activity are given match them correctly
DNA polymerase               	          Source
a. Tma	                                     i. Thermotoga maritima	
b. Deep vent tm	                     ii. Pyrococcus furiosus
c. Tli	                                     iii. Thermococcus litoralis
d. Pfu	                                     iv.  Pyrococcus sp
e. Pwo	                                     v. Pyrococcus woesi
TLS Online TPP Program
                                                    #Question id: 18707
#Unit 13. Methods in Biology
primer dimers are formed when 
 
                                                    TLS Online TPP Program
                                                    #Question id: 18708
#Unit 13. Methods in Biology
PCR can be analysed amplification, Anneleaning, and extension and analysis is called
 
                                                    TLS Online TPP Program
                                                    #Question id: 18709
#Unit 13. Methods in Biology
qPCR can be done by 
i) cyanin dye or SYBR green – to measure amplification
ii) Taqman assay – measure extension
iii) molecular beacons - denaturation
 
                                                    TLS Online TPP Program
                                                    #Question id: 18710
#Unit 13. Methods in Biology
qPCR  graph is drawn between ∆Rn and PCR cycle In this graph CT represents
