PCR efficiency is influenced by several factors, the efficiency declines due to

a) efficiency of amplification declines with an increase in the length of the target sequence.

b) Efficiency of PCR is affected by primer length; it declines if the primers used for PCR arc too long.

c) a temperature higher than the ideal annealing temperature reduces PCR efficiency.

d)  primers used for PCR have complementary regions, dimers will be formed, and PCR efficiency will decline.

TLS Online TPP Program

#Question id: 14079

#Unit 1. Molecules and their Interaction Relevant to Biology

Which DNA is favored in many solutions that are relatively devoid of water with the base pair per turn of

A. B DNA

B. A DNA

C. Z DNA

D. 10 bp per turn

E. 11 bp per turn

F. 12 bp per turn

Which of the following combination is correct?

TLS Online TPP Program

#Question id: 14080

#Unit 1. Molecules and their Interaction Relevant to Biology

 Hairpin and cruciform structures are formed when the DNA sequence have

TLS Online TPP Program

#Question id: 14081

#Unit 1. Molecules and their Interaction Relevant to Biology

pKa of cytosine normally has a value of 4.2 but in hoogsteen triple base pairing this value changes to 

TLS Online TPP Program

#Question id: 14082

#Unit 1. Molecules and their Interaction Relevant to Biology

Conditions where hoogesteen triple base pairing occurs are

A. Presence of alternate GC repeats

B. Presence of only pyrimidines in a strand

C. Presence of only Purines in a strand

D. Acidic pH

E. Basic pH

Choose the option containing correct statement in it

TLS Online TPP Program

#Question id: 14083

#Unit 1. Molecules and their Interaction Relevant to Biology

What components are required for stabilizing DNA double helix

TLS Online TPP Program

#Question id: 14084

#Unit 1. Molecules and their Interaction Relevant to Biology

In DNA helix the angle at which the two sugars protrude from the base pairs (i.e., the angle between the glycosidic bonds) are 

A. 120

B. 36 

C. 240

D. 20

Choose the correct option?