PCR efficiency is influenced by several factors, the efficiency declines due to

a) efficiency of amplification declines with an increase in the length of the target sequence.

b) Efficiency of PCR is affected by primer length; it declines if the primers used for PCR arc too long.

c) a temperature higher than the ideal annealing temperature reduces PCR efficiency.

d)  primers used for PCR have complementary regions, dimers will be formed, and PCR efficiency will decline.

#Question id: 5203

#Unit 11. Evolution and Behavior

The biggest problem with the shotgun technique is its tendency to underestimate the size of the genome. Which of the following might best account for this?

#Question id: 5204

#Unit 11. Evolution and Behavior

Bioinformatics can be used to scan sequences for probable genes looking for start and stop sites for transcription and for translation, for probable splice sites, and for sequences known to be found in other known genes. Such sequences containing these elements are called

#Question id: 5205

#Unit 11. Evolution and Behavior

Why is it preferable to use large computers and databases in searching for individual genes, rather than testing each sequence for possible function?

#Question id: 5206

#Unit 11. Evolution and Behavior

A microarray known as a GeneChip, with most now known human protein coding sequences, has recently been developed to aid in the study of human cancer by first comparing twothree subsets of cancer subtypes. What kind of information might be gleaned from this GeneChip to aid in cancer prevention?

#Question id: 5207

#Unit 11. Evolution and Behavior

Why is it unwise to try to relate an organismʹs complexity with its size or number of cells?

#Question id: 5208

#Unit 11. Evolution and Behavior

Fragments of DNA have been extracted from the remnants of extinct wooly mammoths, amplified, and sequenced. These can now be used to