At times it is observed that non-recombinant plasmids are more than that of recombinant plasmids. Choose the correct statement in regard to it.

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#Question id: 13158

#Unit 2. Cellular Organization

In which process, protein are first encapsulated in a membrane, then the contents of the resulting vesicle are degraded after the vesicle docks with a cytosolic lysosome.

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#Question id: 13159

#Unit 4. Cell Communication and Cell Signaling

How signaling is involved in ensuring that yeast cells fuse only with cells of the opposite mating type?

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#Question id: 13160

#Unit 4. Cell Communication and Cell Signaling

According to the Switching mechanism, monomeric G proteins exist in two states. Match the following states (Column I) with correct mechanism (Column II).

Column I	Column II
A. “ON” state	i. switch I and switch II, are bound to the terminal γ phosphate of GTP through interactions with the backbone amide groups of conserved threonine and glycine residues.
B. “OFF” state	ii. GTPase-activating proteins
	iii. guanine nucleotide exchange factor
	iv. Due to the removal of the γ phosphate by GTPase-catalyzed hydrolysis causes switch I and switch II to relax into a different conformation

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#Question id: 13161

#Unit 4. Cell Communication and Cell Signaling

Which of the following mechanism depends on the activation of heterotrimeric G proteins?

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#Question id: 13162

#Unit 4. Cell Communication and Cell Signaling

In the amoeba Dictyostelium discoideum, cAMP acts as an extracellular signalling molecule and binds to and signals via a G protein–coupled receptor; it is not a second messenger. Amoeba cells were transfected with genes encoding two fusion proteins: a Gα fused to cyan fluorescent protein (CFP), and a Gβ fused to yellow fluorescent protein (YFP). CFP normally fluoresces 490-nm light; YFP, 527-nm light. In which conditions fluorescence

energy transfer occur from CFP to YFP?

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#Question id: 13163

#Unit 4. Cell Communication and Cell Signaling

Following statements are regarding to the interaction between β2-adrenergic receptor and G- protein.