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#Question id: 18808


What would happen if serum is omitted from the ELISA, but all other steps remain same?

#Unit 13. Methods in Biology
  1. Anti-human Ig-conjugate would not bind and be washed away
  2. The OD values would be nearly the same as the assay control
  3. Both (a) and (b)
  4. Anti-human Ig-conjugate would bind non-specifically to the ELISA plate