TLS Online TPP Program

#Question id: 18834


A researcher is trying to alter five successive base pairs in the middle of a PCR amplified DNA fragments. Which technique is the most recommended for performing such experiment? 

#Unit 13. Methods in Biology
  1. DNA ligation
  2. Electrophoretic mobility shift assay (EMSA)
  3. PCR mutagenesis
  4. Restriction enzyme digestion