Nickel-NTA (Ni-NTA) chromatography is a popular affinity chromatography method for the purification of histidine-tagged proteins. However, SDS-PAGE of the eluted protein can show bands in addition to your target protein. How might you improve this purification step?

TLS Online TPP Program

#Question id: 1509

#Unit 4. Cell Communication and Cell Signaling

Cells that are infected, damaged, or have reached the end of their functional life span often undergo "programmed cell death." This controlled cell suicide is called apoptosis. Select the appropriate description of this event on a cell's life cycle.

TLS Online TPP Program

#Question id: 21814

#Unit 12. Applied Biology

A biosensor used for the detection of S-nitrosothiols have immobilized glutathione peroxidase enzyme containing selenocysteine residue that catalytically produces 

TLS Online TPP Program

#Question id: 16487

#Unit 12. Applied Biology

Few limitations regarding organ culture 

It can be maintained only for a few months

Not suitable for biochemical and molecular analyses

For each study, fresh organ cultures have to be initiated

It denatures very easily

Which of the above statements are correct? 

TLS Online TPP Program

#Question id: 5531

#Unit 5. Developmental Biology

The trophoblast forms to the

TLS Online TPP Program

#Question id: 28270

#Unit 5. Developmental Biology

 Increase in cGMP and Ca2+ stimulates flagellar movement in the sperm by activating__ 

a) mitochondrial GTP-generating apparatus 

b) mitochondrial ATP-generating apparatus

c) Na+/K+ ATPase

d) dynein ATPase