Nickel-NTA (Ni-NTA) chromatography is a popular affinity chromatography method for the purification of histidine-tagged proteins. However, SDS-PAGE of the eluted protein can show bands in addition to your target protein. How might you improve this purification step?

#Question id: 739

#Unit 1. Structure and Function of Biomolecules

What is true about the rotation about bonds in a protein backbone?

#Question id: 740

#Unit 1. Structure and Function of Biomolecules

The conformation of the backbone of a polypeptide is described completely by the angle(s) of rotation about which bond(s)?

#Question id: 741

#Unit 1. Structure and Function of Biomolecules

Which of following statements are INCORRECT about secondary structure of proteins

A. Long block of Glu residues segment of the chain will not form an alpha helix at pH 7.0 but it ll take helical confirmation below its pKR value

B. Long block of Lys residues segment of the chain will not form an alpha helix at pH 7.0 but it ll take helical confirmation above its pKR value

C. Long block of Ala residues segment of the chain will not form an alpha helix at pH 7.0 but it ll take helical confirmation below its pKR value

D. Histidine side chain remain uncharged over pH range 3-6

#Question id: 742

#Unit 1. Structure and Function of Biomolecules

Match the following

#Question id: 743

#Unit 1. Structure and Function of Biomolecules

Glycophorin, an integral membrane protein, has a single transmembrane alpha helix. Which of the following idealized hydropathy plots most likely represents the transmembrane nature of glycophorin?

#Question id: 27081

#Unit 1. Structure and Function of Biomolecules