Where in the genome are loxP sites located in a Cre/Lox model?

TLS Online TPP Program

#Question id: 34087

#Unit 3. Fundamental Processes

A DNA containing which an origin of replication undergoing to initiation of replication and further, is digested with restriction endonuclease. Which shape of DNA segment can be found after digestion followed electrophoresis.

A. Y Shape B. Bubble shape C. bubble-to-Y-arc D. Circular

TLS Online TPP Program

#Question id: 34088

#Unit 3. Fundamental Processes

How can the 2D gels identify the DNA intermediates associated with a replication origin? Choose combination of correct statements

A. Y-shaped molecule that has three equal length arms will migrate the most slowly of all such molecules derived from a particular DNA fragment

B. A Y-shaped molecule with two very short replicated arms and a large replicated region will migrate very similarly to the unreplicated version of the same DNA fragment. 

C. Y-shaped molecule that results from the almost completely replicated fragment is similar in shape to a linear molecule two times the size of the unreplicated fragment.

TLS Online TPP Program

#Question id: 34089

#Unit 3. Fundamental Processes

A. Replication Initiation require full methylation of the Dam target sites in the origin

B. Replication Initiation inhibited by hemimethylation of Dam target sites 

C. Replication Initiation inhibited by nonmethylation of Dam target sites 

D. An origin of nonmethylated DNA can function effectively

Choose true and false

TLS Online TPP Program

#Question id: 34090

#Unit 3. Fundamental Processes

A. DnaA is in the active form only when bound to ATP.

B. All three13-bp repeats of origin must be opened for the initiation

C. 9-bp repeats are found outside of 245 bp origin 

D. DnaA has a weak intrinsic activity that converts the ATP to ADP, this is enhanced by a factor termed Had

Choose true and false

TLS Online TPP Program

#Question id: 34091

#Unit 3. Fundamental Processes

DnaA has intrinsic ATPase activity that hydrolyzes ATP to ADP and thus inactivates itself when the initiation stage ends. This ATPase activity is stimulated by 

A. Membrane phospholipids which helps in ATP hydrolysis

B. dsDNA which is substrate for DnaA

C. Membrane phospholipids which provide ATP in exchange of ADP

D. ssDNA which is created in first step

Which of the following is correct combination

TLS Online TPP Program

#Question id: 34092

#Unit 3. Fundamental Processes

A. SeqA binds to hemimethylated DNA and is required for delaying rereplication.

B. As the origins are hemimethylated they bind to the cell membrane and may be unavailable to methylases.

C. The dat locus contains DnaA-binding sites that titrate availability of DnaA protein.

D. Hda protein is recruited to the replication origin to convert DnaA-ATP to DnaA-ADP.

Choose true and false