It is possible to calculate the number of clones that must be present in a gene library to give a probability of obtaining a particular DNA sequence. This formula is:

 

where N is the number of recombinants, P is the probability and f is the fraction of the genome in one insert. Thus for the E. coli DNA chromosome of 5 × 10 ⁶ bp andan insert size of 20 kb ( f = 0.004), the number of clones needed ( N ) would be 1 × 10 ³ ,with a probability of P will be

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#Question id: 11469

#Unit 2. Cellular Organization

plant part not absorb light, which type of plastid it is

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#Question id: 11470

#Unit 2. Cellular Organization

Active form of phytochrome is

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#Question id: 11490

#Unit 2. Cellular Organization

Valinomycin, which is one of the best characterized ionophores, specifically transports

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#Question id: 11491

#Unit 2. Cellular Organization

What is the “signature sequence” that is highly conserved in all K+ channels?

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#Question id: 11492

#Unit 2. Cellular Organization

Which of the following integral membrane protein transport chemotherapeutic drug out of the cell and prevents their accumulation within a tumor and thus blocks their therapeutic effects?

TLS Online TPP Program

#Question id: 11493

#Unit 2. Cellular Organization

Which of the following membrane protein form channel, in the form of gap junctions between cells?