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#Question id: 18956


It is possible to calculate the number of clones that must be present in a gene library to give a probability of obtaining a particular DNA sequence. This formula is:
 
where N is the number of recombinants, P is the probability and f is the fraction of the genome in one insert. Thus for the E. coli DNA chromosome of 5 × 10 6 bp andan insert size of 20 kb ( f = 0.004), the number of clones needed ( N ) would be 1 × 10 3 ,with a probability of P will be

#Unit 13. Methods in Biology
  1. 0.66
  2. 0.77
  3. 0.88
  4. 0.99